; Rodriguez-Bravo, V.; Galsky, M.; Cordon-Cardo, C.; Domingo-Domenech, J. Martarelli, D.; Pompei, P.; Baldi, C.; Mazzoni, G. Mebendazole inhibits growth of human adrenocortical carcinoma cell lines implanted in nude mice. Williamson, T.; Bai, R.Y. Most importantly, daily oral mebendazole prevented established thyroid tumors from metastasizing to the lung. In addition, the tumor vascularity in vivo was quantitated in control and MZ-treated mice using a hemoglobin assay. ; Springer, C.J. B, significant growth inhibition was observed when nu/nu mice were fed 1 mg of MZ every other day. . ; Marshall, C.J. Lung cancer has become the leading cause of cancer death in the world (1). The statements, opinions and data contained in the journal, © 1996-2020 MDPI (Basel, Switzerland) unless otherwise stated. Davis, R.J.; Van Waes, C.; Allen, C.T. NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. You seem to have javascript disabled. This gives rise to the question of whether these mechanisms are directly or indirectly related. ; Chung, J.H. ; Bunz, F.; Riggins, G.J. When administered p.o. Abstract We have found that mebendazole (MZ), a derivative of benzimidazole, induces a dose- and time-dependent apoptotic response in human lung cancer cell lines. This paper follows a comprehensive review, published in 2014 by Pantziarka et al. Aras, S.; Zaidi, M.R. ; Lovat, P.E. The present review summarizes the current literature regarding the use of the anthelmintic mebendazole (MBZ) as a repurposed drug in oncology with a focus on cells resistant to approved therapies, including so called “cancer stem cells”. The present review summarizes the current literature regarding the use of the anthelmintic mebendazole (MBZ) as a repurposed drug in oncology with a focus on cells resistant to approved therapies, including so called "cancer stem cells". Given the low toxicity and published anticancer mechanisms of mebendazole, this novel preclinical study of mebendazole in thyroid cancer has promising therapeutic implications for patients with treatment refractory papillary or anaplastic thyroid cancer. ; Bunz, F. Repurposing the antihelmintic mebendazole as a hedgehog inhibitor. ; Watterson, D.M. . At Hopkins, meanwhile, there is a 6-year study in progress to determine the maximum safe dosage for humans using mebendazole, an indication that the company is taking the potential for cancer treatment by this class of drugs quite seriously. The pharmacokinetics and bioavailability of mebendazole in man: A pilot study using [3H]-mebendazole. Control and MZ-treated cells were washed in cold PBS. ; Confortin, G.; Junqueira, A.V. Cell invasion inhibition (0.085 μM). Learn how to send educational resources to your patient Log in. Human colon cancer cell lines HT29, HCT-8 and SW626, HCT 116 and RKO, Less than 5 μM for all the lines tested and <1 μM for 3 lines. To summarize the study results, we reported descriptive statistics such as the mean and SD. ; Jones, C.M. ; Duhachek-Muggy, S.; Bhat, K.; et al. This includes ascariasis, pinworm disease, hookworm infections, guinea worm infections, hydatid disease, and giardia, among others. Five-μm sections of paraffin-embedded tissue samples were stained with H&E and subjected to immunoperoxidase detection of endothelial cells using a CD31 antibody (18) Both control and treated mice were then monitored for tumor growth, with cross-sectional diameter of the tumors measured externally every 7 days. Although the diverse activities of these compounds have been described at both the biochemical and cellular levels, their molecular mechanism of action has not been explored in detail; when it has been studied, this mechanism has proven to be controversial. . 1C⇓ ; Bouche, G. Drug repurposing in oncology--patient and health systems opportunities. ; Pomeroy, S.L. We use cookies on our website to ensure you get the best experience. Update December 2019: A recent study suggests that for Pancreatic Cancer, two other anti-worm drugs from the same category and used in animals, Parbendazol (brand name Verminum, Worm Guard and Helatac) and Oxibendazole, is more effective compared to Fenbendazole and Mebendazole (Ref.). Arif, S.H. ; Korshunov, A.; Lichter, P.; Taylor, M.D. Braithwaite, P.A. Bars, SE. NSCLC,3 the most common type of lung cancer, has a poor prognosis despite improvements in early diagnosis and treatment. ... MBZ induced a dose- and time-dependent apoptotic response in human lung cancer cell lines, and apoptosis via Bcl-2 inactivation in chemoresistant melanoma cells. ; Silva, A.O. On the basis of the inhibitor profile of both fumarate reductase and glucose uptake, it is apparent that these systems are not specific to BZs, and thus most studies support the hypothesis of microtubule dependence of the action of BZs. The cells were processed for fluorescence-activated cell sorter analysis to determine the cell cycle phase and apoptosis. Chamber assay shows that MZ inhibited capillary formation in A549 cells. The observed safety of the BZs as anthelmintics may also be unrelated to BZ-tubulin binding but rather may be attributable to differences in the metabolic or detoxification pathways as suggested by Nare et al. ; Worden, F.P. 2D)⇓ Sasaki, J.; Ramesh, R.; Chada, S.; Gomyo, Y.; Roth, J.A. Mebendazole, a well-known anti-helminthic drug in wide clinical use, has anti-cancer properties that have been elucidated in a broad range of pre-clinical studies across a number of different cancer types. Location: 2 locations A, A549 cells formed colonies on lung surfaces when injected through the tail vein. ; Montenegro, R.C. Inhibition of MAPK/ERK pathway, induction of apoptosis, synergy with trametinib, Human head and neck squamous cell carcinoma CAL27 and SCC15, Apoptosis induction as a single drug. Although BZs are known as microtubule poisons, a comparison of their relative activity showed that some structural refinement produces BZ derivatives, such as MZ, that are significantly less active against mammalian tubulin. Murine parental or SMO-D477G mutated medulloblastoma. I, A549 cells prelabeled using a fluorescent cell marker were detected on the chamber membranes of control (Con) and MZ-treated (MZ) mice. Induction of, Human GBM14 glioblastoma Murine GL261 glioma, Eight different Acute Myeloid Leukemia cell lines, IC50s for cell viability between 0.07 and 0.26 µM, Degradation of c-MYB and inhibition of its expression. 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